An alternative place with the chromosome eight to possess SH was understood of the assessment the people on screenhouse
With the society Kalarata/NSIC Rc238, a total of four significant QTLs on chromosomes step three, 5, six, eight, and you can 8 was detected getting Sur less than one another tests requirements (Dining table 2). Three tall QTLs was observed getting SH into chromosomes step 1, 3, and seven, of which QTLs to your chromosomes step one and you can step 3 had been perceived in both tests requirements.
This new Sur QTLs qSUR5–step 1 (named as qSUR5–step 1
The tolerant parent Kalarata contributed the SUR QTLs qSUR3–1Rc238-SCR-fourteen, qSUR6–1Rc238-SCR-fourteen, and qSUR7–1Rc238-SCR-14 while the QTLs qSUR5–1Rc238-SCR-14 and qSUR8–1 Rc238-SCR-14 came from susceptible parent NSIC Rc238 under screen house condition at 14 DAS (Table 2). Same five QTLs showed effect on survival at 14 DAS under tray-on-table condition and at 21 DAS in both screening conditions. The largest effect QTL qSUR7–1 (named as qSUR7–1Rc238-SCR-fourteen, qSUR7–1Rc238-TAB-fourteen, qSUR7–1Rc238-SCR-21 and qSUR7–1Rc238-TAB-21) showed a LOD score of to and explained % to % of the phenotypic variation, with an additive effect of % to %, followed by qSUR6–1 (named as qSUR6–1Rc238-SCR-fourteen, qSUR6–1Rc238-TAB-fourteen, qSUR6–1Rc238-SCR-21 and qSUR6–1Rc238-SCR-21), which explained % to % of the phenotypic variation, detected by a LOD score of 5.97 to 6.96, and had an additive effect of 9.79% to%. qSUR3–1 (named as qSUR3–1Rc238-SCR-fourteen, qSUR3–1Rc238-TAB-fourteen, qSUR3–1Rc238-SCR-21 and qSUR3–1Rc238-TAB-21) contributed % to % of the phenotypic variation, with an additive effect of % to %. Rc238-SCR-fourteen, qSUR5–1Rc238-TAB-fourteen, qSUR5–1Rc238-SCR-21 and qSUR5–1Rc238-TAB-21) and qSUR8–1 (named as qSUR8–1Rc238-SCR-fourteen, qSUR8–1Rc238-TAB-fourteen, qSUR8–1Rc238-SCR-21 and qSUR8–1Rc238-TAB-21) from high-yielding susceptible parent NSIC craigslist hookup experience Rc238 contributed 9.76% to % and % to % of the phenotypic variation, with LOD scores of 4.10 to 4.57 and 4.78 to 5.21, respectively (Table 2, Additional file 1: Figure S2).
All SH QTLs were contributed by Kalarata, the parent with anaerobic germination potential. The largest effect QTL identified for SH is qSH1–1 (named as qSH1–1Rc238-SCR-21 and qSH1–1Rc238-TAB-21), which is consistent over both screening conditions. It explained a phenotypic variation of 12.5% to 22.1%, with a LOD score of 5.4 to 9.4. The second largest effect QTL detected for SH was consistent with SUR QTL qSH3–1 (named as qSH3–1Rc238-SCR-fourteen, qSH3–1Rc238-SCR-21 and qSH3–1Rc238-TAB-21,), which was also consistent in both screening conditions. This QTL explained phenotypic variation of 10.1% to14.4%, with a LOD score of 3.7 to 5.8 and an additive effect of 0.84% to 5.17%. QTL qSH7–1Rc238-SCR-21 was identified with a LOD score of 4.6 and explained 10.7% of the phenotypic variation and had an additive effect of 4.57%. It was identified only under screenhouse conditions and was located in a different position from the identified QTL (qSUR7–1) for SUR (Additional file 1: Table S2, Additional file 1: Figure S3A). Under controlled conditions, no QTL was detected for germination, whereas one QTL on chromosome 1 (qSH1-2Rc238-CON-21) was detected for SH with an LOD score of 6.1 that could explain 14.1% of the phenotypic variation (Additional file 1: Table S2). Altogether, the identified QTLs for SUR explained phenotypic variation in the range of % to % while that for the trait SH ranged from 10.1% to 36.5% for the Kalarata/NSIC Rc238 population.
Toward inhabitants Kalarata/NSIC Rc222, a maximum of three extreme QTLs toward chromosomes 3, 6, and you may eight were detected having Sur under each other tests conditions, which have been and consistent with the earliest society (Dining table step three). A couple of high QTLs were recognized to possess SH to the chromosomes step one and you may step three, from which the fresh new QTL with the chromosome step 1, thought of significantly less than both screening standards, was also common with the first population for the very same trait. In every circumstances, mother Kalarata with anaerobic germination prospective discussed the newest knowledgeable alleles having AG (Extra file step one: Dining table S3).